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International Journal Of Horticulture, Agriculture And Food Science(IJHAF)

Determination of the Growth and Bacteriocin Production Kinetics associated with Bacillus pumilus and Bacillus subitlis Isolated from Rastrineobola argentea (Omena)

Juliana Maina Wanjiru , Dr. Julius Maina Mathar , Prof. Gideon Kikuvi M.


International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-1, Issue-3, September - October 2017, Pages 33-41, 10.22161/ijhaf.1.3.7

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This study aimed to determine of the growth and bacteriocin production kinetics of Bacillus pumilus and Bacillus subitlis isolated from Rastrineobola argentea. The study accomplished this goal by focusing on the findings of the existing literature. The findings of the existing literature reveal that B. subtilis and B. pumilus possess a broad-spectrum antimicrobial action against numerous microorganisms including S. aureus, L. plantarían, C. perfringens, L. monocytogenes, B. cereus, E. coli, and L. mesenteroides. The existing literature also reveal that bacteriocins from the two bacteria can survive high temperature of up to 80 0 C for B and 120 0C for B. subtilis. Besides, the existing literature reveal that the microbial action of bacteriocins from the B. subtilis and B. pumilus are not affected is not affected by proteolytic enzymes such as lipase, proteinase, and trypsin among others. While these bacteriocins operate well over a range of pH conditions (pH = 4.0 to 9.0), their optimum action is realized in alkaline and neutral pH conditions (pH =7). In addition the findings of the literature review reveal that bacteriocins from B. subtilis and B. pumilus exhibit maximum antimicrobial action when they are subjected to peptone and glucose concentrations of 0.5% and 0.25% respectively in MRS broth. The existing literature also established that bacteriocins from the two bacteria are tolerant to high salt concentrations of up to 5% for both KCl and NaCl. However, the existing literature reveal that these bacteriocins cannot survive long duration of subjection to U.V light or rays. The researcher recommends the employment of bacteriocins produced by Bacillus pumilus and Bacillus subtilis extracted from Rastrineobola argentea as bio-preservatives, as they are effective.

Bacillus pumilus, Bacillus subitlis, Rastrineobola argentea, Bacteriocins, Growth.

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