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International Journal Of Rural Development, Environment And Health Research(IJREH)

Effects of phosphorus on germination and seedling growth of Cucumis sativus L. in arsenic solution culture

Bayezid M. Khan , Afroza H. Afrin , Mohammad Mosharraf Hossain

International Journal of Rural Development, Environment and Health Research(IJREH), Vol-2,Issue-1, January - February 2018, Pages 48-55, 10.22161/ijreh.2.1.6

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Arsenic (As) is a toxic metalloid which poses a serious threat to the environment and human health. Enrichment of soil with phosphorus (P) is believed to reduce the negative effects of As on plant growth. However, only a few attempts have been conducted towards finding the precise role of P. Τhis study aimed to determine the efficacy of P in reducing the negative effects of As on Cucumis sativus with respect to seed germination rate, seedling growth and biomass production of seedling. The experiment was conducted in petridish under laboratory conditions using P and As solution cultures. Seeds of C. sativus were treated with different concentrations of As (2 ppm, 5 ppm and 10 ppm), with or without addition of P (10ppm). The results revealed that, As solutions reduce the germination rate, seedling growth and biomass production of seedling in different magnitudes, based on the level of concentrations (2ppm, 5 ppm and 10 ppm) in C. sativus. However, addition of P (10 ppm) in As solution cultures (2ppm, 5ppm and 10ppm respectively) increases those parameters significantly in comparison with their corresponding As solutions. In treatments T1 (2ppm As), T3 (5ppm As) and T5 (10ppm As), seedling dry biomasses were 21.1mg, 18.3mg and 13.4mg respectively, while they were 25.6mg, 27.6mg and 20.9mg respectively in T2 (2ppm As+10ppm P), T4 (5ppm As+10ppm P) and T6 (10ppm As+10ppm P). Similar trend was also recorded for germination rate, plumule growth, seedling fresh biomass production, seedling vigor, sturdiness etc. Therefore, it can be recommended to apply P in As solution culture to reduce the negative effects of As on C. sativus growth in the laboratory, that might also be applicable in the field.

Arsenic, Cucumis sativus L, germination, phosphorus, seedling growth, sturdiness, vigor index.

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