Amino acid profile, Antioxidant Activity and flavonoid Content of White Lotus ‘Purain’ from North Bihar, India

B. R. Jana; Manoj Kumar; S.M. Raut

doi:10.22161/ijhaf.8.3.3

Indexing and Abstracting

Amino acid profile, Antioxidant Activity and flavonoid Content of White Lotus ‘Purain’ from North Bihar, India

B. R. Jana , Manoj Kumar , S.M. Raut

International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-8,Issue-3, July - September 2024, Pages 21-26, 10.22161/ijhaf.8.3.3

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Article Info: Received: 23 Jun 2024; Received in revised form: 27 Jul 2024; Accepted: 04 Aug 2024; Available online: 12 Aug 2024

Abstract:

India's national flower is the lotus. A lot of seed is carried by white lotuses. Protein and other bioactive substances are abundant in white lotus seeds. An experiment was carried out at the ICAR-National Research Centre in Makhana in 2023-24 to assess the nutritional quality and anti-oxidant characteristics of white lotus seed, which is abundant during the rainy and autumn seasons in the wetland ecosystem of north Bihar. Seeds of white purain accession were grinded and its flour chemically analyzed using AOAC standard methods. Estimation of amino acids was based on the reaction of free amino acids with phenyl isothiocyanate produces stable derivatives that are then separated using liquid chromatography. Anti-oxidant activities were estimated in spectrophotometric observation by DPPH and FRAP methods, based on electron transfer reactions, which visually results on the reduction of a coloured oxidant. Flavonoids are estimated by UHPLC methods. The white lotus purain seed content 17.8±054 % protein, 1.09±0.07 % fat and 1.94±0.08 % crude fibre. The nutritional study also revealed that the appreciable amount of arginine (12.70 g/kg ) and histidine (3.62 g/kg ) were present in the protein of the dry endosperm of Nelumbium spp. In addition, the lotus seed endosperm has a fair source of quercetin (422.0µg/kg) and kaempferol (200.0 µg/kg) as pure flavonoids which influence the rapid glucose metabolism in human cell. The greatest DPPH and FRAP values were found in seed endosperm of white lotus purain, which had 988.05±2.25 mg AEAC/100g FW and 461.78±1.79 mg AEAC/100g FW, respectively.

Keywords:

White lotus, amino acids, anti-oxidant activity, flavonoids

References:

[1] Arooj, M., Imran, S., Inam‐ur‐Raheem, M., Rajoka, M.S., Sameen, A., Siddique, R., Sahar, A., Tariq, S., Riaz, A., Hussain, A., Siddeeg, A. (2021). Lotus seeds (Nelumbinis semen) as an emerging therapeutic seed: A comprehensive review. Food Science & Nutrition. 7,)3971-87. Available from: https://onlinelibrary.wiley.com/doi/pdf/10.1002/fsn3.2313
[2] Soumya, H., Rachana, S., Jasnoor, K. (2019). A Review on Health Benefits of Lotus Seeds (Nelumbo nucifera). 2019 Dec; 5. 92-96. Available from: https://www.researchgate.net/publication/337856597_A_Review_on_Health_Benefits_of_Lotus_Seeds_Nelumbo_nucifera
[3] Mani, S.S., Subramanian, I.P., Pillai, S.S., Muthusamy, K. (2010). Evaluation of hypoglycemic activity of inorganic constituents in Nelumbo nucifera seeds on streptozotocin-induced diabetes in rats. Biological Trace Element Research. 138 (1), 226-37. Available from: https://link.springer.com/article/10.1007/s12011-010-8614-4
[4] Poornima, P., Weng, C.F., Padma, V.V. (2014). Neferine, an alkaloid from lotus seed embryo, inhibits human lung cancer cell growth by MAPK activation and cell cycle arrest. Biofactors. 40 (1), 121-31. Available from: https://iubmb.onlinelibrary.wiley.com/doi/abs/10.1002/biof.1115
[5] Chakravarthi, P.V., Gopakumar, N., Nair, A.M., Joy, A.D. (2009). Assessment of analgesic activity of red and white lotus seeds (Nelumbo nucifera) in albino rats. Journal of Natural Remedies. 1, 224-7. Available from: https://indianjournalsonline.com/index.php/jnr/article/download/244/244
[6] Rai, S., Wahile, A., Mukherjee, K., Saha, B.P., Mukherjee, P.K. (2006) .Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds. Journal of Ethnopharmacology. 104 (3), 322-7. Available from: https://www.academia.edu/download/47550399/j.jep.2005.09.02520160726-7400-8x3e69.pdf
[7] Makhana side effects [Internet]. Makhana. 2020 [cited 2022Dec12]. Available from: https://makhana.org/makhana-growers/makhana-side-effects/
[8] Rong, Tsao., Raymond, Yang. (2003). Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography. Journal of Chromatography A, 1018, 29–40.
[9] Shahzad, M.A., Ahmad, N., Ismail, T. , Manzoor, M.F. , Ismail, A., Ahmed N., et al. (2021). Nutritional composition and quality characterization of lotus (Nelumbo nucifera Gaertn.) seed flour sup-plemented cookies. Journal of Food Measurement and Characterization, 15 (1), 181-188
[10] Sathithon P., Yan-bin, X. (2012). Effect of sprouting on the chemical and nutritional qualities and phenolic alkaloid content of lotus (Nelumbo nucifera Gaertn.) seeds. Afr. J. Food Sci. 6, 204–211. [Google Scholar]
[11] Brand-Williams, W., Cuvelier, M.E., Berset, C.(1995). Use of free radical method to evaluate antioxi-dant activity. Lebens- Wiss Technol. 28, 25–30.
[12] Benzie, I.F., Strain, J.J.(1996). The ferric reducing ability of plasma (FRAP) as measurement of “antioxidant power” The FRAP assay. Anal Biochem. 239, 70–6.
[13] A.O.A.C.(2016). Official Methods of Analysis, Association of Official Analytical Chemists. 20 th Edition, 923.03 -984.13, Washington, DC, USA, 2016.
[14] A.O.A.C.,(2016). Official Methods of Analysis. Association of Official Analytical Chemists. Washington, DC, USA.
[15] Ranganna, S. (1997). Handbook of Analysis and Quality Control for Fruit and Vegetable Products. Tata McGraw Hill Publishing Co. Ltd., New Delhi,; 190-210.
[16] Bidlingmeyer, B.A., Cohen S,A. , Taroin T.L. , Frost, B. (1987). A new rapid high sensitivity analysis of Amino acids in food type samples. Journal Assoc. Official Anal. Chem., 70, 241-247.
[17] Kumar, Lokendra., Singh, A.K. Bhat, B.P. (2016). Nutritional status of recently developed Makhana (Gorgon Nut) variety - Swarna Vaidehi. Journal of AgriSearch, 3(4), 199-205.
[18] Jana , B.R., Kumar, Ujjwal, I.S.Singh, and Anuradha Srivastava.(2021). Makhana: A Nutritious and fabulous functional food. Biotica Research Today 2021, 3(7), 605-607
[19] Jana, B.R. and Idris, Md. (2018). Anti-aging amino acids in Euryale ferox (Salisb.). Advances in Plant and Agriculture Research, 8(1), 43-48 DOI: 10.15406/apar.2018.08.00289.
[20] Shahzad, M.A., Ahmad, N., Ismail, T., Manzoor, M.F., Ismail, A., Ahmed, N. et al.(2021). Nutritional composition and quality characterization of lotus (Nelumbo nucifera Gaertn.) seed flour sup-plemented cookies. Journal of Food Measurement and Characterization 15(4), 1-8
[21] Yi Zhang, Xu Lu, Shaoxiao Zeng, Xuhui Huang, Zebin Guo, Yafeng Zheng, Yuting Tian, Baodong Zheng,(2015). Nutritional composition, physiological functions and processing of lotus (Nelumbo nucifera Gaertn.) seeds: a review, Phytochemistry Reviews, 10.1007/s11101-015-9401-9, 14( 3), 321-334
[22] Zeng, H.Y, Cai, L.H., Cai, X.L., Wang, Y.J. Li ,Y.Q.(2012). Amino acid profiles and quality from lotus seed proteins. J Sci Food Agric. 93(5), 1070-5. doi: 10.1002/jsfa.5848. Epub 2012 Dec 4. PMID: 23208980.
[23] Zhu M, Liu T and Guo M (2016) Current Advances in the Metabolomics Study on Lotus Seeds. Front. Plant Sci. 7:891. doi: 10.3389/fpls.2016.00891

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Amino acid profile, Antioxidant Activity and flavonoid Content of White Lotus ‘Purain’ from North Bihar, India

Abstract:

Keywords:

References: