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International Journal Of Rural Development, Environment And Health Research(IJREH)

Radiobiological and Biophysical Showing of Venom of Macrovipera Lebetina Obtusa

Topchiyeva Shafiga , Abiyev Huseyn


International Journal of Rural Development, Environment and Health Research(IJREH), Vol-1,Issue-3, September - October 2017, Pages 1-12,

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Research objective was studying of influence of ecological factors on radiobiology-cal and biophysical properties of venom of Transcaucasian vipera Macrovipera lebetina obtusa. By method of atomic absorption spectrometry, metal ions Cr, Pb, Cd and Zn was revealed in samples of vipera venom. γ – radiospectrometric studies have shown that the venom samples also contain radionuclides as Ra228, Ra226, K40 and 137 Cs. Analysis of the data shows that the venom of Viperas caught from different regions of the Republic, are almost indistinguishable for the content of radionuclides. All samples of the venom have a lower content of Ra228.Generalizing results on research of infra-red, visible and ultra-violet spectra of absorption of vipera venom, it is possible to ascertain that infra-red, visible and ultra-violet spectra of standard samples of vipera venom and samples irradiated by -radiation are studied and systematized. The received experimental data testify to perspectivity of spectral methods at biophysical and biochemical researches of both snake venom , and preparations on its basis, showing informative at studying of structural changes under the influence of ecological factors.It was established that the radiation dose (up to 1.35 kGy dose) for 3 minutes did not cause structural changes in the samples venom of vipera, but rather contribute to the stabilization of both toxicity and pharmacological activity while increasing the shelf life of aqueous solutions of vipera venom. As a result of experimental studies, a negative effect of metal ions and radiation on the biochemical and biophysical indices of snake venom

snake venom, Macrovipera lebetina obtusa, metal ions, radiation.

[1] Ahunov А. “Some physico-chemical and biological properties of venom proteases of the Central Asian vipera”. Uzb. Bio. Journal., 1974, 2, pp.75-76.
[2] Abiyev H.A., Topchiyeva Sh.A. “Influence to small dozes radiation on spectral characteristics and pharmacological properties of venom tranmscaucasian viper Vipera lebetina obtusa.” The 35 th Annual Meeting of the European Radiation Research Society and The 4 th Annual Meeting of the Ukrainian Society for Radiation Biology., Kiyev, Ukraine, 2006, p. 223
[3] Abiyev H.A., Topchiyeva Sh.A., Rustamov V.R. “Radiating sterilization of the venom of snake.” Fourth Euroasian Conference Nuclear science and its application , Bakı, 2006, p. 178.
[4] Berdiyeva А.Т. “Pathophysiological aspects of the toxicological action of snake venoms”, Ashgabat, Ylym, 1990, 163 p.
[5] Ishaki Y.B. “Effect of venom vipera on postoperative course in the extirpation of the tonsils.” Bulletin of Otorhinolaryngology, 1959, 5, pp. 44-47.
[6] Mitelman L.М. “Comparative data on the impact on the process of blood clotting venoms of different snakes. Questions Hematology radiobiol of magnetic fields,” Tomsk, 1965, pp. 299-302
[7] Said-Aliyev S.А. Amphibians and Reptiles of Tajikistan, Donish, Dushanbe, 1979, pp. 139-140.
[8] Topchiyeva Sh.A., Abiyev H.A. “Comparative effect of soil pollution of Absheron peninsula in Azerbaijan on the chemical composition of venom of Transcaucasian vipera -Vipera lebetina obtusa.” Bilgi journal , Bakı, 2004, 4, pp. 74-78.
[9] Topchiyeva Sh.A., Abiyev H.A. “The action of gamma irradiation on the spectral characteristics of vipera venom.” J. Izvestia NASA, Series of Biological Sciences, Baku, 2006, 5-6, pp. 138-143.
[10] Samel M., Subbi J., Siigur J., Siigur E. “Biochemical characterization of fibrinogenolytic serine proteinases from Vipera lebetina snake venom.” J. Toxicon, 2002. 40 (1), pp. 51-54.
[11] Sánchez, E.E., Ramírez, M.S., Galán, J.A. “Cross reactivity of three antivenoms against North American snake venoms”, J.Toxicon, 2000, (241), pp. 315-320.
[12] Topchiyeva Sh.A. “Chromatographic and spectral characteristics of snake venom, proteins and products of metabolism of ones.” J. Farmakom, Kharkov. 2002, (4), pp. 75-81.
[13] Topchiyeva Sh.A. “Fluorescent probes in snake venom investigation”. J. Farmakom, Kharkov, 2002, (3), pp. 174.-179.
[14] Topchiyeva Sh.A., Iskenderov T.M., Jabbarov R.B., Musayeva N.N. “Influence of the ecological factors to number Vipera lebetina obtusa and chemical composition of venom”. Proceedings of the First International Conference on Environmental Research and Assessment, Bucharest, Romania, 2003, pp. 34-37.
[15] Topchiyeva Sh.A., Abiyev H.A., Mehrabova M.A.” Investigation of Snake Venom of Vipera Lebetina Obtusa”. 5th WSEAS International Conference on Nanotechnology (NANOTECHNOLOGY '13) Cambridge, UK, 2013, pp. 29-34.
[16] Topchiyeva Sh.A., Abiyev H.A., Mehrabova M.A. “Impact of environmental pollution factors on Macrovipera lebetina obtuse venom.” WSEAS International Conf. On Mathematical and Techniques in Engineering and Environmental Science., Cataniya, Sicily, Italy, 2011, pp: 75-78.