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International Journal Of Rural Development, Environment And Health Research(IJREH)

Isolation of biologically active and anticoagulant components from the venom of the snake Macroipera lebetina obtusa and honey bee Apis mellifera Caucasica

Sh.A. Topchiyeva , E.T. Babayev

International Journal of Rural Development, Environment and Health Research(IJREH), Vol-1,Issue-3, September - October 2017, Pages 51-57, 10.22161/ijreh.1.3.6

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The paper presents experimental data on the separation, identification and isolation of anticoagulant components of the snake venom and of the honey bee Apis mellifera Caucasica, harvested from the ecologically clean zone of Azerbaijan. The protein components of venom snake and bee venom with molecular masses 20 kD, 32kD, 35, 79 , 101, 132 kD and 41kD, 20 kD, 15 kD, corresponding to glycoprotein, toxic component, growth factor, protease that activates the clotting factor of the blood, glycoproteins and hyaluronidase and phospholipase was isolated from venom by the method of gel chromatography on a column with Sephadex G-750 eluting with 0.4 M sodium phosphate buffer, followed by spectrophotometric measurement of the unit optical density of the fractions at =280 nm on a Hitachi-557 spectrophotometer.

snake, venom, Macroipera lebetina obtuse, honey bee, Apis mellifera Caucasica, anticoagulant.

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