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International Journal Of Rural Development, Environment And Health Research(IJREH)

Isolation Characterization and Screening of fungal Lipase from oil contaminated Soil: An approach of best from Waste

Debosmita Sikdar , Ivy Kanungo , Monim Ul Islam

International Journal of Rural Development, Environment and Health Research(IJREH), Vol-4,Issue3, May - June 2020, Pages 60-66, 10.22161/ijreh.4.3.1

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Present scenario demands a more sustainable, ecofriendly and economic measures globally to deal with the growing problems of environmental issues. The main goal of this work is to opt for such ideas and technologies which involve cleaner and greener procedures for utilizing waste materials for deriving value added products. The soil pertaining to the areas of oil mills contains densely population of various microbes’, especially fungal origin. These microbes are rich in lipase content (due to oil source). Thus in this we isolated fungal colonies from this oil rich soil, cultured in laboratory, fermented them under various conditions to extract fungal enzyme i.e. lipase and then used it for further applications. Lipases are highly versatile and industrially important enzymes. Deriving the lipases from waste soil is the main attraction of this work and is a venture strategizing the “best from waste” approach.

fungal Lipase, oil contaminated Soil, waste materials.

[1] M. Blamey, F. Fischer, H. P. Meyer, F. Sarmiento, M. Zinn, Enzymatic Biocatalysis in Chemical Transformations: A Promising and Emerging Field in Green Chemistry Practice, Biotechnology of Microbial Enzymes, Production, Biocatalysis and Industrial Applications, 2017, 347-403.
[2] K. de G. Daiha, R. Angeli, S. D. de Oliveira, R. V. Almeida, Are Lipases Still Important Biocatalysts,2015, 10(6), e0131624.
[3] C. D. Anobom, A. S. Pinheiro, R. A. De-Andrade, E. C. G. Aguieiras, G. C. Andrade, M. V. Moura, R. V. Almeida, D. M. Freire, From Structure to Catalysis: Recent Developments in the Biotechnological Applications of Lipases, BioMed Research International, 2014, 2014, Article ID 684506, 11 pages.
[4] M. Kapoor, M. N. Gupta, Lipase promiscuity and its biochemical applications, Process Biochemistry, 2012, 47(4), 555–569.
[5] R. Fernandez-Lafuente, Lipase from Thermomyces lanuginosus: uses and prospects as an industrial biocatalyst, Journal of Molecular Catalysis B: Enzymatic, 2010, 62, (3-4), 197–212.
[6] A. K. Singh, M. Mukhopadhyay, Overview of fungal lipase: a review, Applied Biochemistry and Biotechnology, 2012, 166(2), 486-520.
[7] A. Gog, M. Roman, M. Toşa, C. Paizs, F. D. Irimie, Biodiesel production using enzymatic transesterification: current state and perspectives, Renewable Energy, 2012, 39(1), 10–16.
[8] Entry for mustard oil in the USDA National Nutrient Database for Standard Reference, Release 22.
[9] L. Fjerbaek, K. V. Christensen, B. Norddahl, A review of biodiesel production using enzymatic transesterification, Biotechnology and Bioengineering, 2009, 102(5), 1298–1315.
[10] F. R. de Souza, M. Gutterres, Application of enzymes in leather processing: a comparison between Brazilian Journal of Chemical Engineering, 2012, 29(03), 473 - 481.