IJMPD | The Detection of Testicular Protein Expression of Rats (Rattus norvegicus) after being treated with Ethanol Extract of Fenugreek Seeds

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The Detection of Testicular Protein Expression of Rats (Rattus norvegicus) after being treated with Ethanol Extract of Fenugreek Seeds

Zaneb M. Benmansur , Agung J. Sitasiwi , Kasiyati Kasiyati

International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-4,Issue-6, November - December 2020, Pages 36-43 ,

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Article Info: Received: 25 Sept 2020; Received in revised form: 07 Nov 2020; Accepted: 12 Nov 2020; Available online: 10 Dec 2020

Abstract:

One of the testes' primary functions is to produce the haploid germ cells needed for sexual reproduction. Good vitality can be achieved through a healthy biological pattern and require lasting persistence throughout a productive male's life. This study aims to detect protein expression in rats' testes after being treated with ethanol Fenugreek seeds. As many as 32 male rats (Rattus norvegicus), aged 2-3 months (250-300 g) without anatomical abnormalities were used as experimental animals. They were divided into four groups: P0 as a control group was treated with normal drinking water, while groups of P1 to P3 were given 500, 1000, and 1500 mg kg-1 body weight/day of ethanol extract from Fenugreek seeds. The experiment of testicular protein expression was conducted with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The testes data were analyzed by One-way Analysis of Variance (ANOVA) and the Duncan Mean Range Test. The study concluded that Fenugreek's effect is believed to have an inducing impression on protein synthesis in the cells making up the testes. Besides, body weight, body weight gain, testicular weight, and testicular protein showed no significant difference (P> 0.05).

Keywords:

body weight, Fenugreek, protein expression, rats, testis.

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