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International Journal Of Medical, Pharmacy And Drug Research(IJMPD)

Rat Lung Histology After Exposure to Cigarette Smooke Made from Brugmansia suaveolens Plant

Nurmadina Najib , Tyas Rini Saraswati , Sunarno Sunarno


International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-4,Issue-6, November - December 2020, Pages 44-49 ,

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Article Info: Received: 15 Oct 2020; Received in revised form: 05 Nov 2020; Accepted: 19 Dec 2020; Available online: 27 Dec 2020

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Brugmansia suaveolens contains tropane alkaloids such as atropine, hyoscyamine and scopolamine which have anticholinergic properties. This study aims to analyze the effects of exposure to cigarette smoke made from amethyst leaves and flowers on the lungs based on histopathological features. This study used 20 male Rattus norvegicus strains of Wistar, consisting of 4 treatments and 5 replications. This study used a completely randomized design (CRD) with treatment, namely P0: rats without exposure to cigarette smoke (control), P1: rats were exposed to cigarettes from amethyst leaves, P2: rats were exposed to cigarettes from amethyst flowers, P3: rats were exposed to cigarettes from amethyst mix of leaves and amethyst. The treatment was carried out for 21 days. The parameters observed in this study were alveolar septal destruction, increased black particles in lung tissue, alveolar diameter, and alveolar epithelial cell thickness. Data were analyzed using the normality Shapiro-Wilk test because the sample size was ≤50, followed by thetest method one way Anova. The results showed that there was a significant difference (P> 0.05) in the variable diameter of the alveolar epithelial cell thickness, namely treatment P1 to P0. Exposure to cigarette smoke from leaves, flowers, leaf mixtures and mountain amethyst (Brugmansia suaveolens) can cause disturbances in lung structure and function characterized by destruction of the alveolar septum, inflammatory cell infiltration, the emergence of black particles, and an increase in the diameter and thickness of the alveolar epithelial cells. Exposure to cigarette smoke from mountain amethyst leaves (Brugmansia suaveolens) has the most toxic effect on the lungs than exposure to cigarette smoke from flowers or a mixture of the two.

Brugmansia suaveolens, cigarettes, lung histopathology.

[1] Jayawickreme, K.P., Janaka, K.V.C., Subasinghe, S.A.S.P, “Unknowing ingestion of Brugmansia suaveolens leaves presenting with signs of anticholinergic toxicity”: a case report. Journal of Medical Case Report, vol. 13, 2019, pp.322.
[2] Nugroho, M.A.A, “Uji Potensiasi Narkose/Depresan Natrium Thiopental Dengan Ekstrak Etanol Kecubung Gunung (Brugmansia candida Pers.) terhadap Mencit Putih Jantan” Fakultas Farmasi Universitas Muhammadiyah Surakarta, 2007.
[3] Anggraini, D, “Kebijakan ASEAN dalam Menanggulangi Penyalahgunaan Narkotika dan Obat-Obatan Berbahaya di Asia Tenggara”, Jurnal Analisis Hubungan Internasional, vol. 5, pp.40.
[4] Doan, V.U., Wu, L.M., Phua, H.D., Rojas, M.B., Chang, C. Yang, C.C, “Datura and Brugmansia plants related antimuscarinic toxicity: an analysis of poisoning cases reported to the Taiwan poison control center”, Clinical Toxicology, vol. 57, 2019, pp.246-253.
[5] Drope, J., Eriksen, M., Schlunger, N., Farhad, I, “The Tobacco Atlas Fifth edition”, USA: American Cancer Society, 2014.
[6] Rahimah, B.S., Sastramihardja, S., & Sitorus, D. T., “Efek Antioksidan Jamur Tiram Putih pada Kadar Malondialdehid dan Kepadatan Permukaan Sel Paru Tikus yang Terpapar Asap Rokok”, MKB, vol. 42, 2010, pp.195-202.
[7] Abbas, A., Aster, J., Kumar, V, “Buku Ajar Patologi Robbins”, Edisi 7, EGC, Jakarta, 2013.
[8] Anisa, I., N., dan Andreanus A., S, “Uji Efek Bronkodilator Ekstrak Air Daun dan Bunga Kecubung Gunung (Brugmansia Suaveolens Bercht & Presl)”, Laporan Penelitian, Jurusan Farmasi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jenderal Achmad Yani. Cimahi, 2013.
[9] Aisyah, S.I., Balqis, U., Eko, K.F, “Histopatologi Jantung Tikus Putih (Rattus norvegicus)Akibat Pemberian Minyak Jelantah”, Medika Veterinaria, vol.8, pp.87-90, 2014.
[10] Adrian, J., A., U., Kusumorini, N., Agungpriyono, S., , Sismin, A., S., Boediono, A, “Analisis Mikroskopis Paru-Paru Tikus Jantan (Rattus norvegicus) yang Terpapar Asap Rokok”, Jurnal Biologi Edukasi, vol.10, pp.8-11, 2018.
[11] Shraideh, Z., Badran, D.H., Al-Awaida W, “Effects of cigarette smoking on histology of trachea and lungs of albino rat”, Res. Opin. Anim. Vet. Sci, vol. 3, pp.356-62, 2013.
[12] Luliana, S., Purwati, N., U., Manihuruk, K., N, “Pengaruh Cara Pengeringan Simplisia Daun Senggani (Melastoma balabathricum L.) Terhadap Aktivitas Antioksidan Menggunakan Metode DPPH ( 2,2 difenil-1-pikrihidrazil)”, Pharm sci res, vol.3, 2016.