IJMPD | Polygonum Persicaria (Linn.) and its Active Principle have a hepatoprotective and antioxidant effect on carbon tetrachloride-induced toxicity in rats

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Polygonum Persicaria (Linn.) and its Active Principle have a hepatoprotective and antioxidant effect on carbon tetrachloride-induced toxicity in rats

Mohd Shafi Dar , Deepak Kumar Mittal , Shazia Tabasum , Rafeeq Ahmad Najar , Abu Tahir

International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-6,Issue-2, March - April 2022, Pages 1-7 , 10.22161/ijmpd.6.2.1

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Article Info: Received: 30 Jan 2022; Received in revised form: 20 Feb 2022; Accepted: 01 Mar 2022; Available online: 08 Mar 2022

Abstract:

The aim of this analysis was to see whether the aqueous extract of the roots of Polygonum persicaria (PP) and its active principle, Tannic Acid (TA), had a hepatoprotective and antioxidant effect in rats provided Carbon tetrachloride (1.5 ml/kg, i.p). Twenty albino wistar rats were divided into five groups: control, CCl4-induced hepatotoxicity, hepatotoxicity with Polygonum persicaria and Tannic acid, and a normal group given 100 mg/kg silymarin. After 14 days, the rats were sacrificed. Toxicity testing was carried out on 12 rats. They were randomly allocated to one of three groups: control, Polygonum persicaria 200 mg/kg (B.wt), and Tannic acid 200 mg/kg (B.wt). The amounts of liver homogenate enzymes (glutathione peroxidase, glutathione-S-transferase, glucose-6-phosphatase dehydrogenase, and glutathione reductase enzymes) were greatly restored by extracts of PP and TA at the tested concentrations, supporting the biochemical results. Tannic acid, in contrast to Polygonum persicaria, tends to have a greater liver defensive role toward carbon tetrachloride-induced hepatotoxicity, as well as antioxidant properties and mild anticancer activity against cell viability at higher concentrations. The histological alterations in the liver indicated the injury. Polygonum persicaria & its active principle Tannic acid has strong antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity, as demonstrated by these observations.

Keywords:

Polygonum persicaria, Tannic acid, antioxidant, Hepatoprotective, Carbon tetrachloride, Anti cancerous, Silymarin, & Histopathology.

References:

[1] A. Sehrawat and S. Sultana, (2006). “Evaluation of possible mechanisms of protective role of Tamarix gallica against DEN initiated and 2- AAF promoted hepatocarcinogenesis in male Wistar rats,” Life Sciences, vol. 79, no. 15, pp. 1456–1465.
[2] Askar MA, Sumathy K, ZaheerBaquer N (1996). Regulation and properties of purified glucose6-phosphate dehydrogenase from rat brain. Indian J BiochemBiophys; 33: 512- 518.
[3] Bandyopadhyay U, Das D, Banerjee KR (1999). Reactive oxygen species: Oxidative damage and pathogenesis. Curr Sci. 77: 658-66.
[4] Bhuwan Chandra Joshi, Atish Prakash, Ajudhia N. Kalia (2015). Hepatoprotective potential of antioxidant potent fraction from Urtica dioica Linn. (Whole plant) in CCl4 challenged rats; Toxicology Reports 2 (2015) 1101–1110. http://dx.doi.org/10.1016/j. toxrep.2015.07.020.
[5] C. Loguercio and A. Federico, (2003). “Oxidative stress in viral and alcoholic hepatitis,” Free Radical Biology & Medicine, vol. 34, no. 1, pp. 1–10.
[6] Duwiejua M, Zeitlin IJ, Gray AI, Waterman PG (1999). The anti-inflammatory compounds of Polygonum bistorta: Isolation and characterization. Planta Medica; 65: 371-374.
[7] Feingold BF (1986). The Feingold Handbook. Alexandria, Va.: The Feingold Association of the United States.
[8] Gonzalez-Burgos E, Gomez-Serranillos MP (2012). Terpene compounds in nature: a review of their potential antioxidant activity. Curr. Med. Chem. 19(31):5319-41.
[9] Gupta et al., (2011). Visual estimation and spectrophotometric determination of tannin content and antioxidant activity of three common vegetable; Vol. 2(1): 175-182.
[10] Mamillapalli A, Ganeshbabu D, Swarnalatha P (2013). Antioxidant, antiproliferative and antimicrobial activities of Zingiberroseum rocs., an endangered medicinal plant. Int J Scientific Res. 2(7):25-30.
[11] Matthaus B (2002). Antioxidant activity of extracts obtained from the residue of different oil seeds. J Agric Food Chem. 49: 3444-3452.
[12] Memy H Hassan, Mohamed Edfawy, Ahmed Mansour and Abdel-Aziz Hamed (2011). Antioxidant and antiapoptotic effects of capsaicin against carbon tetrachloride-induced hepatotoxicity in rats; Toxicology and Industrial Health 28(5): 428–438. DOI: 10.1177/07482337114 13801.
[13] N. Aghel, I. Rashidi, and A. Mombeini, (2007). “Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in mice,” Iranian Journal of Pharmaceutical Research, vol. 6, no. 4, pp. 285–290.
[14] Ostapowicz G., Fontana R.J., Schiodt F.V., Larson A., Davron J.T., Steven H.B., Timothy M., Reish J (2002). Results of a prospective study of acute liver failure at 17 tertiary care centers in the United States. Ann Intern Med; 137: 947–954.
[15] Paglia DE, Valentine WN (1967). Studies on qualitative and quantitative characterization of glutathione peroxidase.J Lab clin Med; 70: 158-169.
[16] Recknagel RO, Glende EA, Jr Dolak JA, Walter RL (1989). Mechanism of carbon tetrachloride toxicity. PharmacolTher. 43: 139-54.
[17] Rose MH, Sudhaa PN and Sudhakar K (2014).: Effect of antioxidants and hepatoprotective activities of methanol extract of beet root (Beta vulgaris L.) against carbon tetrachloride induced hepatotoxicity in rat models. Int J Pharm Sci Res; 5(6): 2546- 55.doi: 10.13040/IJPSR.0975-8232.5 (6).2536-45.
[18] Samina Akhter, Md. Atiar Rahman, Jannatul Aklima, Md. Rakibul Hasan, and J. M. Kamirul Hasan Chowdhury (2015). Antioxidative Role of Hatikana (Leea macrophylla Roxb.) Partially Improves the Hepatic Damage Induced by CCl4 in Wistar Albino Rats; Hindawi Publishing Corporation BioMed Research International. Article ID 356729, 12 pages http://dx.doi.org/10.1155/2015/356729.
[19] Sharma A., Chakraborti K.K., Handa S.S. (1991). Anti-hepatotoxic activity of some Indian herbal formulations as compared to silymarin.Fitoterapia; 62: 229-235.
[20] Snedecor GW, Cochran WG (1994). Statistical analysis (8th edn). Lowa State University Press: Ames, IA; 217-236.
[21] Subramonium A., Pushpangadan P. (1999). Development of Phytomedicines for liver diseases. Indian J Pharmacology; 31: 166175.
[22] Tayarani I, Cloez I, Clement M, Bourre JM (1989). Antioxidant enzymes and related free elements in aging brain plexus capillaries and chloroid.J Neurochem; 53: 817-824.
[23] Venukumar MR and Latha MS (2002). Hepatoprotective effect of the methanolic extract of Curculigoorchioides in CCl4- treated male rats. Indian J Pharmacol; 34: 269- 75.