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International Journal Of Medical, Pharmacy And Drug Research(IJMPD)

Microbiological Assessment and Storage Quality of Expressed Breast Milk

Ifeanyi O. C. Obiajuru , Chidinma A. Ikpeama , Jacinta C. Elo – Ilo Elo – Ilo

International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-1,Issue-2, July - August 2017, Pages 5-9 ,

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The microbiological and storage quality of expressed human breast milk was studied between July and December, 2016. One hundred and twenty working class lactating mothers and thirty lactating mothers visiting Imo State University teaching Hospital Orlu for various health challenges were recruited for the study. They were requested to express 60ml of their breast milk into sterile containers. The milk sample collected from each mother was distributed 10ml into each of 3 sterile containers. One set was heated at 100OC for 1hour in a water bath, 1 set was stored in a refrigerator at – 4OC for 5 days and 1 set was stored on the bench at ambient temperature without any treatment. 0.1ml of each sample was inoculated on laboratory culture media before commencement of storage and 2hours, 6hours, 12 hours, 24 hours and 5days post storage. Eight genera of bacteria: Stapylococcus aureus, Streptococcus viridians, Diphtheroides, Escherichia coli, Klebsiella species, Lactobacillus species, Pseudomonas species and Salmonella species, were isolated from expressed human breast milk samples. The most prevalent bacterium in the milk samples was Staphylococcus epidernidis, followed by Escherichia coli. The least prevalent bacteria were Pseudomonas aeruginosa, Salmonella species and Diphtheroides. No bacterium was isolated from milk samples heated at 100OC and stored in a refrigerator. The total heterotrophic bacterial counts of the milk samples collected from healthy working mothers ranged from 3.2 x 103 to 8.2 x 103cfu/ml, while that of health challenged mothers ranged from 4.3 x 103 to 1.6 x 104cfu/ml. As shown, the bacterial counts of the samples. Out of 30 samples collected from health – challenged mothers, 9 (30%) had total heterotrophic bacteria count ranging from 1.2 x 104 to 1.6 x 104cfu/ml, 21 (70%) had total bacteria count ranging from 4.3 x 103 to 8.6 x 103cfu/ml. Analysis of the data using chi square showed significant difference (p < 0.05) in the total heterotrophic bacterial count of breast milk between healthy working mothers and health challenged mother.

Microbiological, Storage, Quality, Expressed – breast milk.

[1] Asquith, M. T. and Harrod, J. R (1979): Reduction of bacterial contamination in banked human milk. Journal of Paeditrics. 95: 993 - 994
[2] Chen, A. and Rogan, W. J (2004): Breast feeding and the risk of neonatal death in the United States. Pediatrics. 113: e435 – e439
[3] Chesbrough M (2002): Medical Laboratory Manual for Tropical Countries. ELSA with Tropical Health Technology Butterworth Heinemann Ltd Oxford 1:. 605 - 607
[4] Deodhar, L. and Joshi, S. (1991): Microbiological study of breast milk with special reference to its storage in milk bank. Journal of Postgraduate medicine. 37(1): 14 – 16
[5] Ghogan (2013): breast milk contains over 700 bacteria species. www.livescience.com
[6] Gastelum, D. T., Dassey, D, Mascola, L AND Yasuda L. M. (2005): Transmission of community associated methicillin resistant Staphylococcus aureus from breast milk in neonatal intensive care unit. Paediatric Infectious disease Journal.24: 1122 - 1124
[7] Hanson, L. A. and Korotkova, M (2002): The role of breast feeding in prevention of neonatal infection. Semin Neonatal. 7: 275 – 281
[8] Hylander, M. A., Strobino, D. M. and Dhanireddy, R (1998): Human milk feeding and infection among very low birth weight infants. Paediatrics. 102: E38
[9] Kotiw, M, Zhang, G. W., Daggard, G., Reoss – Levy, E., Tapsall, J. W. and Numa, A (2003): Late onset and recurrent neonatal Group B Streptococcal disease associated with breast milk transmission. Paediatric Dev. Path, 6: 251 - 256
[10] Novak, F. R., Da Silva, A. V., Hagler, A. N. and Figueiredo, A M. S (2000): Contamination of expressed human breast milk with an epidemic multi – resistant Staphylococcus aureus clone. Journal of Postgraduate medicine. 37(1):
[11] Obiajuru, I. O. C. and Ozumba, U. C. (2009): Laboratory Methods for Medical Microbiology and Parasitology. Lifeway Amalgamations, Owerri. ISBN: 25-79-91-07 p. 183
[12] Pronczuk, J. Akre, Moy, G and Vallenas, C (2002): Global perspectives in breast milk contamination: infectious and toxic hazards. Environmental Health Perspective 110: A349 – A351
[13] Qutaishat, S., Stemper, M., Spencer, S. et al., (2003): Transmission of Salmonella enterica serotype Typhimurin DT104
[14] Roy C.C and Lescop I. (1979):Human milk banking : high rate of interest for a still uncertain credit balance. American Journal of Dis. Child. 133: 255 – 256
[15] Sasson, A. (1990): Feeding Tomorrow’s World. UNESCO / CTA. Paris p. 805
[16] Svabic - Vlahovic, M., Pantic D, Pavicic, M and Gryner, A H. (1998): Transmission of Listeria monocytogenes from mother’s milk to her babies and puppies. Lancet 2: 1201
[17] UNICEF (1993): In Sasson A. (1990): Feeding Tomorrow’s World. UNESCO / CTA. Paris p. 805
[18] WHO (1985): The Quantity and Quality of breast milk: Report on the collaborative study on Breast feeding. World Health Organization. Geneva p. 148
[19] Widdows, S. T. and Lowenfed, M. F. (1983): Biochemical Journal 27: 1400