• editor.aipublications@gmail.com
  • Track Your Paper
  • Contact Us
  • ISSN: 2456-8015

International Journal Of Medical, Pharmacy And Drug Research(IJMPD)

Detection molecular for microdeletions of the Y chromosome in IVF technique

Mustafa Faeq Kazem Hussein , Israa Sami Othman , Mohammed Abdulfatah Al-Ayashn , Abbas Abdul Wahhab Jumaah Al-Salihi , Khaleel Ibrahim Ismael


International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-7,Issue-1, January - February 2023, Pages 25-29 , 10.22161/ijmpd.7.1.4

Download | Downloads : | Total View : 462

Article Info: Received: 15 Jan 2023; Received in revised form: 12 Feb 2023; Accepted: 22 Feb 2023; Available online: 28 Feb 2023

Share

Y chromosome microdeletion tests are the basis of success IVF technique to disclose the real problem for patients males (oligozoospermia) to reproductive treatment. The present study to showed the relationship between microdeletion and IVF technique, then early detection of Y chromosome microdeletion is auxiliary to venereal treatment, particularly in those young patients with AZFc deletion. The study was used 65 patients, males (oligozoospermia ) and ten normal males, as control. DNA was recovered and treated, which was used for Y (CMD) through PCR SYBR Green real-time PCR for the amplification of three loci AZFa, AZFb, and AZFc regions. Our results showed only six samples (9 %) of single micro-deletions in the Y chromosome. Finally, the early diagnosis is very important for oligozoospermia patients whose will undergo the IVF technique. Kew word: Y chromosome, IVF technique, SYBER Green, Oligozoospermia.

IVF technique, Y chromosome, DNA, Microdeletion, AZFa, AZFb, and AZFc.

[1] Foresta C, and Moro E, Ferlin A. Prognostic value of Y deletion analysis. The role of current methods. Hum Reprod. 2001;16 (8):1543–7.
[2] Foresta C, and Moro E, Ferlin A. Y chromosome micro deletions and alterations of spermatogenesis. Endocr Rev. 2001;22 (2):226–39.
[3] Vogt PH, Edelman A, Kirsch S, et al. Human Y chromosomeazoospermia factors (AZF) mapped to different sub regions inYq11. Hum Mol Genet. 1996;5 (7):933–43.
[4] Buch B, Galán JJ, Lara M, Ruiz R, Segura C, Real LM,Martínez-Moya M, Ruiz A. Scanning of Y-chromosomeazoospermia factor loci using real-time polymerase chainreaction and melting curve analysis. Fertil Steril. 2003;80:907-13.
[5] Laboratoire de Biologie de la Reproduction, HoÃpital Nord, 42055 Saint Etienne, 2Laboratoire de GeÂneÂtique Mole Âculaire and Faculte de. Y chromosome micro deletion screening in infertile men in France: survey of French practice based on 88 IVF centres. Human Reproduction Vol.19, No.4 pp. 788±793, 2004.
[6] Yuen R, Merkoulovitch A, MacDonald JR, Vlasschaert M, LoK, Grober E, Marshall CR, Jarvi KA, Kolomietz E, and SchererSW. Development of a high-resolution Y-chromosome microarray for improved male infertility diagnosis. Fertil Steril.2014;101:1079-85.e3.
[7] Zaimy M, Kalantar SM, Sheikhha MH, Jahaninejad T,Pashaiefar Ghasemzadeh J, Zahraei M. The frequency of Yq micro deletion in azoospermic and oligospermic Iranian infertile men. Iran J Reprod Med. 2013;11:453-8.
[8] Kozina V, Cappallo-Obermann H, Gromoll J and Spiess AN. A One-Step Real-Time Multiplex PCR for Screening Y Chromosomal Micro deletions without Downstream Amplicon Size Analysis. Plos One. 2011; 6:e23174.
[9] Zhu XB, Gong YH, He J, et al. Multicenter study of Y chromosome micro deletions in 1,808 Chinese infertile males using multiplex and real-time polymerase chain reaction. Andrologia 2017;49:12726–32.
[10] Said M.M., Fahmi A.A., Hemeda H.M., Aly B., AbdelAal R.H., Nasr S.M., El Gamal M., Fawzy I.O., El Tayebi H.M. and Abdelaziz A.I. Detection of Ychromosome micro deletions in recurrent abortions among Egyptian females using SYBR Green Real Time PCR. Journal of American Science 2013;9 (9):150-156]. (ISSN: 1545-1003).
[11] Tatiana Puga Torres1, Xavier Blum Rojas1, Medardo Blum Narváez1, Edith López Montanero2, Alexandra NarváezSarasti2. Triplex Real-time Polymerase Chain Reaction Optimization for AZF Y-chromosome Micro deletion Analysis. JBRA Assisted Reproduction .2015;19 (2):37-43
[12] Fu L, Xiong DK, Ding XP, et al. Genetic screening for chromosomal abnormalities and Y chromosome microdeletions in Chinese infertilemen. J Assist Repro’d Genet 2012;29:521–7.
[13] Dincer M, Karahasanoglu A, Uzun Cilingir I, et al. Frequency of Y chromosome microdeletions and chromosomal abnormalities I infertile Turkish men. Genet Couns 2013;24:431–4.
[14] Ting Liu, MD, PhDa,b,∗, Yu-Xin Song, MDa, Yong-Mei Jiang, MD, PhDa . Early detection of Y chromosome micro deletions in infertile men is helpful to guide clinical reproductive treatments in southwest of China. Medicine. 2019; 98:5.