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International Journal Of Medical, Pharmacy And Drug Research(IJMPD)

Bronchus Histology of Wistar Rats (Rattus norvegicus L) that are exposed to the cigarette smokes given with Angel's Tears leaves (Brugmansia suaveolens) smoke treatment

Tanty. F Audria , Sri. Isdadiyanto , Sunarno Sunarno


International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-5,Issue-2, March - April 2021, Pages 4-9 , 10.22161/ijmpd.5.2.2

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Article Info: Received: 03 Dec 2020; Received in revised form: 19 Jan 2020; Accepted: 21 Feb 2021; Available online: 25 Mar 2021

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Angel's tears (Brugmansia suaveolens Bercht. & Presl) contain tropane alkaloids, such as atropine, hyoscyamine and scopolamine which have anticholinergics potential. This study aims to analyze the effect of Angel's tears leaf smoke on the thickness of the mucous-submucosal membrane and the number of bronchial goblet cells of white rats induced by cigarette smoke exposure. Two months old male Wistar rats (Rattus norvegicus L.) were used (average weight 200 g). This study was an experimental study with a completely randomized block design consisting of 5 treatments and 4 replications, namely K0: negative control, K1: Positive control was given exposure to 1 cigarette / day, P3: K1 + Aminophylline 8.1 mg / 200 g BW, and two treatment groups (P1, P2) were given the smoke of Angel's tears with different doses. White rat bronchi were prepared for histological observation using the paraffin method and Hematoxylin-Eosin staining. Data analysis using ANOVA followed by Duncan's Multiple Range Tests with a confidence level of 95%. The results showed that the smoke of Angel's tears can reduce the thickness of the mucous-submucous membrane and the number of goblet cells in white rats. This study provides information to the public that Angel's tears can be used as an alternative treatment for respiratory disease.

Brugmansia suaveolens, cigarette smoke, goblet cells, mucosal-submucosal membrane thickness

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