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International Journal Of Medical, Pharmacy And Drug Research(IJMPD)

Anti-bacterial and Anti-oxidant Studies on the Stem Bark Extracts of Prunusafricana

Teshale Ayano Begeno

International Journal of Medical, Pharmacy and Drug Research(IJMPD), Vol-4,Issue-3, May - June 2020, Pages 16-23 , 10.22161/ijmpd.4.3.2

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Prunusafricana belongs to the Rosaceae family. It is a geographically wide spread tree to forest habitats of the African continent. P. africana is one of the most popular plants in traditional medicine for treating various ailments. It is mainly used to treat benign prostate hyperplasia (BHP). The study was aimed to evaluate the anti-bacterial and anti-oxidant activities of stem bark extract from P. africana. The air dried and powdered plant material (300 g) was first soaked with 400mLofn-hexane for 72 hours and yielded 1.5g of n-hexane extract. Residue was soaked with 400mL of ethyl acetate for 48hours and afforded 4.5g of ethyl acetate extract. Finally, residue was soaked with 400mLof methanol and yielded 12.5g of methanol extract. The ethyl acetate extract showed inhibition zones of 16mm and 11mm against Escherichia coli and Staphylococcus aureus, respectively.The extracts also showed encourage results of DPPH radical scavenging activity at various concentrations. The ethyl acetate extract of P. africana of stem bark showed promising activity against E. coli, ATCC24534 and S.aureus,ATCC26452. Anti-oxidant activities also were shown prospective result, selectively at lowest concentration and lowest absorbance. This means the result of the study was confirmed that the lowest concentration of 12.5mg/mL and absorbance of 0.208the scavenging activity was 77.6%, while at the highest concentration of 200mg/mL and absorbance of 0.288the scavenging activity was 68.9%.

stem bark; medicine; ailments;anti-bacterial; anti-oxidant; inhibition;Escherichia coli; Staphylococcus aureus.

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