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International Journal Of Horticulture, Agriculture And Food Science(IJHAF)

Stimulating the Viability of Bifidobacterium spp. in Synbiotic Fermented Milk by Co-culturing with Lactobacillus paracasei 441 and Inulin

Amira A. Ayad , Deiaa Gad El-Rab , Ghada El-Kherbawy , Shafika Zakic , Leonard Williams

International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-2,Issue-5, September - October 2018, Pages 174-181, 10.22161/ijhaf.2.5.2

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The aim of this study is to examine the ability of three Bifidobacterium (bifidum, breve, and infantis) and Lactobacillus paracasei 441 strains to survive at different pH values, and different bile salts concentrations for 3h, also to determine the stimulatory effect of L. paracasei 441 strain in combination with inulin on the viability of Bifidobacterium strains in fermented milk during 15 days at 4°C. Each tested strain was cultured with and without L. paracasei 441 in fermented milk, then each treatment was split into three groups [0,1.0, and 3.0 inulin (w/v %)] and incubated at 37°C for 3h. Our results showed that the bacterial populations of Bifidobacterium strains co-culturing with L. paracasei 441, and 1% Inulin) were significantly (P > 0.05) higher than in the control samples [without (L. paracasei 441 and inulin). Our results reveal that the presents of (L. paracasei 441, and inulin) was stimulated the viability of Bifidobacterium strains during storage condition.

Bifidobacterium, Inulin, Low pH and Low bile salts, Stimulating, and Synbiotic fermented milk.

[1] Ayad, Amira A. (2016). Survival and Viability of Freeze/Spray-Dried Lactobacillus ssp. Grown in Date Palm (Phoenix dactylifera L.) Base Medium. North Carolina Agricultural and Technical State University,
[2] Ayad, Amira A, El-Rab, Deiaa Gad, Shahbazi, Abolghasem, Worku, Mulumebet, Schimmel, Keith, Ejimakor, Godfrey, . . . Ibrahim, Salam A. (2016). Using Date Palm (Phoenix dactylifera L.) by-products to Cultivate Lactobacillus reuteri spp. Journal of Food Research, 5(5), 77.
[3] Ayad, Amira Aly Fahmy. (2011). Formulation of special diets for preschoool children.
[4] Barakat, Olfat S, Ibrahim, GA, Tawfik, NF, El-Kholy, WI, & El-Rab, Gad DA. (2011). Identification and probiotic characteristics of Lactobacillus strains isolated from traditional Domiati cheese. International Journal of Microbiology Research, 3(1), 59-66.
[5] Champagne, Claude P, Ross, R Paul, Saarela, Maria, Hansen, Ken Flemming, & Charalampopoulos, Dimitris. (2011). Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices. International Journal of Food Microbiology, 149(3), 185-193.
[6] de Souza Oliveira, Ricardo Pinheiro, Perego, Patrizia, de Oliveira, Maricê Nogueira, & Converti, Attilio. (2012). Effect of inulin on the growth and metabolism of a probiotic strain of Lactobacillus rhamnosus in co-culture with Streptococcus thermophilus. LWT-Food Science and Technology, 47(2), 358-363.
[7] Florence, Ana Carolina Rodrigues, de Oliveira, Maricê Nogueira, Delile, Armelle, & Béal, Catherine. (2016). Survival of Bifidobacterium strains in organic fermented milk is improved as a result of membrane fatty acid composition. International Dairy Journal, 61, 1-9.
[8] Goddik, Lisbeth. (2012). Sour cream and crème fraiche. Handbook of Animal-Based Fermented Food and Beverage Technology, 1, 235.
[9] Granato, Daniel, Branco, Gabriel F, Cruz, Adriano Gomes, Faria, José de Assis Fonseca, & Shah, Nagendra P. (2010). Probiotic dairy products as functional foods. Comprehensive Reviews in Food Science and Food Safety, 9(5), 455-470.
[10] Granato, Daniel, Branco, Gabriel F, Nazzaro, Filomena, Cruz, Adriano G, & Faria, Jose AF. (2010). Functional foods and nondairy probiotic food development: trends, concepts, and products. Comprehensive Reviews in Food Science and Food Safety, 9(3), 292-302.
[11] König, Helmut, & Fröhlich, Jürgen. (2017). Lactic acid bacteria. In Biology of Microorganisms on Grapes, in Must and in Wine (pp. 3-41): Springer.
[12] Krzeminski, Alina, Großhable, Katja, & Hinrichs, Jörg. (2011). Structural properties of stirred yoghurt as influenced by whey proteins. LWT-Food Science and Technology, 44(10), 2134-2140.
[13] Mehanna, Nayra SH, Tawfik, Nabil F, Salem, Moussa ME, Effat, Baher AM, & Gad El-Rab, DA. (2013). Assessment of potential probiotic bacteria isolated from breast milk. Middle-East Journal of Scientific Research, 14(3), 354-360.
[14] Meyer, D, Bayarri, S, Tárrega, A, & Costell, E. (2011). Inulin as texture modifier in dairy products. Food Hydrocolloids, 25(8), 1881-1890.
[15] Patel, Anil K, Singhania, Reeta R, Pandey, Ashok, & Chincholkar, Sudhir B. (2010). Probiotic bile salt hydrolase: current developments and perspectives. Applied biochemistry and biotechnology, 162(1), 166-180.
[16] Ranadheera, RDCS, Baines, SK, & Adams, MC. (2010). Importance of food in probiotic efficacy. Food Research International, 43(1), 1-7.
[17] Rivera-Espinoza, Yadira, & Gallardo-Navarro, Yoja. (2010). Non-dairy probiotic products. Food Microbiology, 27(1), 1-11.
[18] Roberfroid, Marcel, Gibson, Glenn R, Hoyles, Lesley, McCartney, Anne L, Rastall, Robert, Rowland, Ian, . . . Stahl, Bernd. (2010). Prebiotic effects: metabolic and health benefits. British Journal of Nutrition, 104(S2), S1-S63.
[19] SERT, DURMUŞ, Akin, Nihat, & Dertli, Enes. (2011). Effects of sunflower honey on the physicochemical, microbiological and sensory characteristics in set type yoghurt during refrigerated storage. International journal of dairy technology, 64(1), 99-107.