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International Journal Of Horticulture, Agriculture And Food Science(IJHAF)

Molecular Identification of Bulinus Species in Ogun State, South-West Nigeria and Observations on Snail Infections with Schistosome

J.B. Ajayi , S.O. Sam-Wobo , A.O. Agbeyangi

International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-1,Issue-1, May - June 2017, Pages 15-20,

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The study considers the distribution of a small sample of 100 Bulinus snails, across 8 localities within Ogun State, Nigerian. Snails were identified using a molecular method of fragment and restriction profiles obtained from ribosomal internal transcribed spacer (its) amplicons. The results showed that the majority of Bulinus samples tested belonged to the species Bulinustruncatus while only one was Bulinusglobosus. The use of Rsa1 restriction endonuclease to cleave the ribosomal its of Bulinus, as a method of species identification, was adopted for the majority of samples, this being a quicker and cheaper method better suited to small laboratory environments. Polymerase chain reaction (PCR) amplification of the schistosome Dra1 repeat within each of the collected Bulinus samples was employed to determine the extent and distribution of infected snails within the sample areas. Successful amplification of the Dra1 repeat demonstrated that 23% of snails were infected with schistosome

Bulinustruncatus, Bulinusglobosus Schistosomahaematobium, PCR-RFLP, schistosome infection.

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