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International Journal Of Horticulture, Agriculture And Food Science(IJHAF)

Molecular Identification of Bulinus Species in Ogun State, South-West Nigeria and Observations on Snail Infections with Schistosome

J.B. Ajayi , S.O. Sam-Wobo , A.O. Agbeyangi


International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-1,Issue-1, May - June 2017, Pages 15-20,

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The study considers the distribution of a small sample of 100 Bulinus snails, across 8 localities within Ogun State, Nigerian. Snails were identified using a molecular method of fragment and restriction profiles obtained from ribosomal internal transcribed spacer (its) amplicons. The results showed that the majority of Bulinus samples tested belonged to the species Bulinustruncatus while only one was Bulinusglobosus. The use of Rsa1 restriction endonuclease to cleave the ribosomal its of Bulinus, as a method of species identification, was adopted for the majority of samples, this being a quicker and cheaper method better suited to small laboratory environments. Polymerase chain reaction (PCR) amplification of the schistosome Dra1 repeat within each of the collected Bulinus samples was employed to determine the extent and distribution of infected snails within the sample areas. Successful amplification of the Dra1 repeat demonstrated that 23% of snails were infected with schistosome

Bulinustruncatus, Bulinusglobosus Schistosomahaematobium, PCR-RFLP, schistosome infection.

[1] Agi, P.I. (1995) Vesical schistosomiasis at Odau village in Ahoada local government area, rivers State, Nigeria. West African Journal of Medicine 14: 6-10.
[2] Akogun OB, Akogun MK. 1996.Human behavior, water usage and schistosomiasis transmission in a small settlement near Yola, Nigeria. Ann Trop Med Parasitol1996; 90: 303-311.
[3] Brown, D. S. Freshwater Snails of Africa and Their Medical Importance. In: (2nd edition), Taylor and Francis, London 1994; pp: 609.
[4] Cowpear SG. (1973). Bilharziasis (Schistosomiasis) in Nigeria. Ann Trop Med Parasitol., 57: 307- 322.
[5] Driscoll AJ, Kyle JL (2005). Remais J. Development of a novel PCR assay capable of detecting a single Schistosomajaponicumcercaria recovered from Oncomelaniahupensis. Parasitol., 131: 497– 500.
[6] Ekpo UF and Mafiana, CF. (2004). Epidemiological studies of urinary schistosomiasis in Ogun State, Nigeria: Identification of high-risk communities. Nigerian Journal of Parasitology, 25: 111-119
[7] Emejulu A.C, Alabaronye FF, Ezenwaje HMG, Okafor FC. (1994). Investigation into the Prevalence of Urinary Schistosomiasis in the Agulu Lake Area of Anambra State, Nigeria. J Helminthol., 68: 119-123.
[8] Hamburger J, Weil M, Turetzky T, et al, (1989). Identification of snails infected withSchistosomes by ELISA employing monoclonal antibodies: Schistosomamansoni in laboratory snails (Biomphalariaglabrata) and in field snails (Biomphalariapfeifferi) from Kenya. Amer J Trop Med Hygiene.,40: 613–619.
[9] Hamburger J, He N, Abbasi I et al. (2001). Polymerase chain reaction assay based on a highly repeated sequence of Schistosomahaematobium: a potential tool for monitoring schistosome-infested water. Amer J Trop Med Hygiene., 65: 907– 911.
[10] Hamburger J, Hoffman O, Kariuki HC, et al. (2004). Largescale, polymerase chain reaction-based surveillance of Schistosomahaematobium DNA in snails from transmission sites in coastal Kenya: a new tool for studying the dynamics of snail infection. Amer J Trop Med Hygiene., 71: 765–773.
[11] Hertel J, Kedves K, Hassan AH, et al. (2004). Detection of Schsitosomamansonicercariae in plankton samples by PCR. ActaTropica., 91: 43–46.
[12] Kristensen, T.K. (1987). A field guide to African freshwater snails. 2nd edn. pp. 1–51. Charlottenlund, Danish Bilharziasis Laboratory.
[13] Mafe MA, StammTVon, Utizinger J, N’Goran EK. Control of urinary schistosomiasis: an investigation into the effective use of questionnaires to identify high-risk communities and individuals in Niger State, Nigeria. Journal of Tropical Medicine and International Health 2000; 5: 53–63.
[14] Okoli, C.G. &Iwuala, M.O. (2004). The prevalence, intensity and clinical signs of urinary schistosomiasis in Imo state, Nigeria. Journal of Helminthology 78, 337–342.
[15] Rabello A, André Pontes L, Dias-NetoE.(2002). Recent Advances in the Diagnosis of Schist soma Infection: the Detection of Parasite DNA. Memoirs de Institut Oswaldo Cruz. 97: 171–172.
[16] Stothard JR, Hughes S, Rollinson D. (1996). Variation within the ribosomal internal transcribed spacer (ITS) from freshwater snails of the genus Bulinus. ActaTropica. 61: 19–29.
[17] Sturrock RF, Diaw OT, Talla I, et al. (2001). Seasonality in the transmission of schistosomiasis and in populations of its snail intermediate hosts in and around a sugar irrigation scheme at Richard Toll, Senegal. Parasitol., 123: 77–89.
[18] World Health Organization (WHO). (2002). Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee. World Health Organ Technical Report Series 2002; 912: 2–3.