• editor.aipublications@gmail.com
  • Track Your Paper
  • Contact Us
  • ISSN: 2456-8635

International Journal Of Horticulture, Agriculture And Food Science(IJHAF)

Molecular Diversity Analysis of Some Local Ginger (Zingiber officinale) Genotypes Using RAPD Markers

M.E. Hoque , F.T. Zohura

International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-3,Issue-1, January - February 2019, Pages 20-28, 10.22161/ijhaf.3.1.3

Download | Downloads : 6 | Total View : 1301


Ginger (Zingiber officinale) rhizomes have been widely used as a spice and flavoring agent in foods and beverages in Bangladesh as well as in all over the world for its economical and medicinal values. The present investigation was undertaken for the assessment of 13 local ginger genotypes collected from different region of Bangladesh through 7 RAPD primers. Genomic DNA was extracted from ginger genotypes using CTAB method. A total of 34 distinct and differential amplification bands ranging from 150-1200 bp were observed with an average of 1.14 polymorphic bands per primer. The overall gene diversity was detected 0.8052 and the value of PIC was detected 0.7532. The RAPD marker generate enough polymorphism for possible use in diversity studies through cluster analysis and principal component analysis (PCA). PCA classified 13 ginger genotypes into four groups and showed in two dimensional scatter plot. The genetic similarity coefficients among genotypes ranged from 0.103 to 0.654. Cluster analysis based on Jaccard’s similarity-coefficient using UPGMA grouped the genotypes into two clusters: Cluster A and Cluster B. The cluster ‘A’ had only one genotype Kaptai local and the second cluster ‘B’ had rest of twelve genotypes. The prevalence level of polymorphism in the local genotypes of ginger will help to breeders for ginger improvement program.

Ginger, Molecular diversity, Polymorphism, RAPD.

[1] Barnes, J., Anderson, L.A., Phillipson, J.D. (2002). Herbal Medicines, 2nd ed. Phar- maceutical Press, London Grayslake, UK. p. 243.
[2] BBS 2017, Statistical Year Book of Bangladesh, Bureau of Statistics Ministry of Planning, Government of the People’s Republic of Bangladesh, Dhaka.
[3] Bennett, M. D. and Smith J. B. (1991). Nuclear DNA amounts in angiosperms. Philos. Trans. R. Soc. London. B 334: 309-345.
[4] Choudhury A. K., Firoz Z. A. and Hoque F. M. E. (1998). Performance of ginger legume inter cropping at different spacing of ginger in hilly region. Bangladesh J. Agril. Res. 23 (1): 133-142.
[5] Cooper, H.D., Spillane, C. and Hodgkin, T. (2001). Broadening the genetic base of crops: an overview. In: Cooper, H.D., C. Spillane and T. Hodgkin (eds.) Broadening the Genetic Base of Crop Production, CABI Publishing, UK. pp. 1-23.
[6] Das, A. B., Mukherjee A. K. and Das P. (2001). Molecular phylogeny of Heritiera aiton (Sterculiaceae), a tree mangrove: variation in RAPD markers and nuclear DNA content. Botanical J. Linn. Soc. 136: 221-229.
[7] Garner-Wizard M., Milot, S. H. B., Neustadt, E. J., Oliff, H. S., Oppel, M., Rapp, C. and Webb, D. (2006). Ginger-an herbal medicinal product with broad anti-inflammatory actions. Journal of Medicinal Food. 8(2): 125–132.
[8] Earl, D. A. (2012). STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation genetics resources. 4: 359-361.
[9] Evanno, G., Regnaut, S. and Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol. Ecol.14: 2611–2620.
[10] Frey, J.E. Frey, B., Sauer, C. and Kellerhals, M. (2004). Efficient low cost DNA extraction and multiple fluorescent PCR method for marker assisted selection in breeding. Plant Breed.123:554-557
[11] Gepts, P. (1993). The use of molecular and biochemical markers in crop evolution studies. In: M.K. Hecht (Ed), Evol. Biol., Plenum Press, New York. 27: 51-94.
[12] Karp, A., Kresovich, S., Bhat, K., Ayad, W., and Hodgkin, T. (1997). Molecular tools in plant genetic resources conservation: a guide to the technologies. IPGRI. Rome, Italy.
[13] Mia, M. S., Patwary, A. K., Hassan, L., Hasan, M. M., Alam, M. A., Latif, M. A., Alam, M. M and Puteh, A. B. (2014). Genetic Diversity Analysis of Ginger (Zingiber officinale) Genotypes Using RAPD Markers. Life Sci. J. 11(8): 90-94.
[14] Nei, M. (1972). Genetic distance between populations. Am Nat. 106: 283-292.
[15] Palai, S.K. and Rout, G.R. (2007). Identification and genetic variation among eight varieties of ginger by using random amplified polymorphic DNA markers. Plant Biotech. 24: 417–420.
[16] Rodriguez, J. M., Berke, T., Engle L., and Nienhuis J. (1999). Variation among and within Capsicum species revealed by RAPD markers. Theor. Appl. Genet. 99: 147-156.
[17] Rout, G.R., Das, P., Goel, S., Raina, S.N., (1998). Determination of genetic stability of micropropagated plants of ginger using random amplified polymorphic DNA (RAPD) markers. Botanical Bulletin of Academia Sinica.39 (1): 23–27.
[18] Sweetman, S.C. (2007). Martindale: The Complete Drug Reference, 35th ed. Phar- maceutical Press, Chicago, London. p. 3322.
[19] Tosti, N. andNegri, V. (2002). Efficiency of three PCR- based markers in assessing genetic variation amon cowpea (Vignaungiculatasppungiculata) landraces. Genome45: 268-275.
[20] Welsh, J. and McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18: 7213-7218.
[21] Williams, J. G. K., Kubelik, A. E., Levak, K.J., Rafalski, J. A. and Tingey, S.C. (1990): DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res.28: 6531-6535.
[22] Yeh, F.C., Yang, RC. and Boyle, T. (1999): POPGENE, version 1.31. Microsoft windows based freeware for population genetic analysis. University of Alberta, Canada.