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International Journal Of Horticulture, Agriculture And Food Science(IJHAF)

Molecular Characterization of Yeast (Saccharomyces cerevisiae) Strains by Using Molecular Marker

A.S. Pawar , Kunvar Gyanendra Kumar , R. P. Singh


International Journal of Horticulture, Agriculture and Food science(IJHAF), Vol-8,Issue-3, July - September 2024, Pages 57-59, 10.22161/ijhaf.8.3.8

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Article Info: Received: 16 Aug 2024; Received in revised form: 17 Sep 2024; Accepted: 23 Sep 2024; Available online: 28 Sep 2024

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Molecular markers reveals polymorphism at the DNA level. Molecular characterization of Yeast (Saccharomyces cerevisiae) Strains with RAPD Molecular markers. The genetic diversity allelic variations among these strains were estimated by using RAPD molecular markers. Twenty Eight RAPD primers were screened out of them eleven primers had shown amplification of which AB1-15, 1283, OPB12, OPO4, SC1 and OPB01 these six primers showed 100% polymorphism. Out of ten wine yeast strains studied at molecular level, two strains showed maximum similarity i.e. 79 % between them viz. NCIM 3045 and 3200. Genetic diversity was analyzed based on data obtained by 11 RAPD primers. Most of the primers were found 85.71 to 100% polymorphic in nature. The diversity at molecular level was analyzed with elucidian distance of 0.40. molecular markers hold great promise due to their high efficiency, adequate accuracy, and good reproducibility

DNA, Genetic variation, Molecular markers, PCR and Yeast.

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