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International Journal Of Forest, Animal And Fisheries Research(IJFAF)

Effect of Neem (Azadirachta indica) leaf Supplementation on Reproductive Performance of Lactating Sahiwal Cows

Kavita Khosla Chatley , Dhirendra Bhonsle , Ranjana Sinha , Nishma Singh , Rupal Pathak

Article Info: Received: 16 May 2026; Received in revised form: 15 Jun 2026; Accepted: 18 Jun 2026; Available online: 25 Jun 2026

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DOI: 10.22161/ijfaf.10.2.4

Journal : International Journal Of Forest, Animal And Fisheries Research(IJFAF)

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The present study was conducted to evaluate the effect of neem (Azadirachta indica) leaf supplementation on reproductive performance of lactating Sahiwal cows. Twelve lactating Sahiwal cows were randomly allocated into two groups comprising a control group (T0) fed a basal diet and a treatment group (T1) supplemented with neem leaf at 200 mg/kg body weight/day for 90 days. Reproductive performance was assessed using first postpartum heat period, calving-to-conception interval, and conception rate. The mean first postpartum heat period was numerically lower in neem-supplemented cows (132.83 ± 19.81 days) than in control cows (157.20 ± 22.37 days). Similarly, the calving-to-conception interval was reduced from 166.00 ± 21.22 days in the control group to 114.67 ± 5.54 days in the neem-supplemented group. Conception rate remained comparable between the two groups (50.00%). However, the observed differences were statistically non-significant (P > 0.05). The numerical improvements in reproductive performance may be attributed to the antiparasitic, antioxidant, immunomodulatory, and protein-sparing effects of neem bioactive compounds, which potentially improve metabolic status and support reproductive recovery during the postpartum period. It was concluded that dietary neem leaf supplementation showed a favourable trend toward improved reproductive performance in Sahiwal cows, although further studies involving larger sample sizes and longer supplementation periods are required to confirm these effects.

Neem leaf, Sahiwal cows, reproductive performance, postpartum heat, conception interval, conception rate.

[1] Borman, A., Islam, M. F., Rahman, M. S., Hoque, M. F., Kispotta, S. and Haque, M. A. (2016). Effects of periparturient anthelmintic treatment on milk yield and quality in dairy cows. Asian Journal of Medical and Biological Research, 2(3), 409–413. https://doi.org/10.3329/ajmbr.v2i3.30111
[2] Chandrawathani, P., Chang, K. W., Nurulaini, R., Waller, P. J., Adnan, M., Zaini, C. M., Jamnah, O., Khadijah, S. and Vincent, N. (2006). Daily feeding of fresh neem leaves (Azadirachta indica) for worm control in sheep. Tropical Biomedicine, 23(1), 23–30.
[3] El-Zaiat, H. M., Elshafie, E. I., Al-Marzooqi, W. and Dughaishi, K. A. (2022). Effects of neem (Azadirachta indica) leaf powder supplementation on rumen fermentation, feed intake, apparent digestibility and performance in Omani sheep. Animals, 12(22), 3146. https://doi.org/10.3390/ani12223146
[4] Gross, S. J., Ryan, W. G. and Ploeger, H. W. (1999). Anthelmintic treatment of dairy cows and its effect on milk production. Veterinary Record, 144(21), 581–587. https://doi.org/10.1136/vr.144.21.581
[5] Harmon, R. J. (1994). Physiology of mastitis and factors affecting somatic cell counts. Journal of Dairy Science, 77(7), 2103–2112. https://doi.org/10.3168/jds.S0022-0302(94)77153-8
[6] Ibrahim, N. H., Nouh, R. R., Helal, A. and Nayel, U. A. (2024). Effects of yeast culture additives on growth performance, blood metabolites and puberty age of cows' heifers. Scientific Journal of Agricultural Sciences, 6(4), 171–184. https://doi.org/10.21608/sjas.2024.330707.1469
[7] Kaplan, R. M. (2004). Responding to the emergence of multiple-drug resistant Haemonchus contortus: Smart drenching and FAMACHA©. College of Veterinary Medicine, University of Georgia, Department of Medical Microbiology and Parasitology, Athens.
[8] Kumar, N., Rao, T. K. S., Varghese, A. and Rathor, V. S. (2013). Internal parasite management in grazing livestock. Journal of Parasitic Diseases, 37(2), 151–157. https://doi.org/10.1007/s12639-012-0215-z
[9] Makkar, H. P. S. (2003). Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research, 49(3), 241–256. https://doi.org/10.1016/S0921-4488(03)00142-1
[10] Njoga, U. J., Jaja, I. F., Onwuka, O. S., Ilo, S. U., Eke, I. G., Abah, K. O., Oguejiofor, C. F. and Ochiogu, I. S. (2022). Reproductive effects of medicinal plant (Azadirachta indica) used as forage and for ethnoveterinary practices: New insights from animal models. Challenges, 13(2), 40. https://doi.org/10.3390/challe13020040
[11] NRC (2001). Nutrient Requirements of Dairy Cattle. 7th revised edition. National Research Council, National Academies Press, Washington DC.
[12] Raj, D. N., Ramana, J. V., Rao, S. B. N., Dinesh Kumar, D., Suryanarayana, M. V. A. N., Ravindra Reddy, Y. and Prasad, K. S. (2016). Effect of incorporation of detoxified karanja (Pongamia pinnata) and neem (Azadirachta indica) seed cakes in total mixed rations on milk yield, composition and efficiency in crossbred dairy cows. Indian Journal of Animal Sciences, 86(4), 489–492. https://doi.org/10.56093/ijans.v86i4.57809
[13] Ramesh, K. S., Susan, E. P. and Devanand, F. (2011). Bioactive constituents and antimicrobial activity of cell cultures of Azadirachta indica. International Journal of Pharmaceutical and Biological Sciences, 2(3), 617–628.
[14] Sarker, M. A. S., Khan, M. H., Rashid, M. and Islam, A. (2016). Effect of Azadirachta indica and Annona reticulata leaf as natural anthelmintics and their effects on performances of zebu cow under subsistence farming condition in Bangladesh. International Journal of Pharmaceutical and Chemical and Biological Sciences, 6(1), 16–21.
[15] Singh, K., Mishra, K. K., Shrivastav, N., Mishra, S. K., Singh, A. K., Jha, A. K., Tiwari, N. and Ranjan, R. (2019). Therapeutic efficacy of indigenous polyherbal formulation on milk pH, somatic cell count and electrical resistance profile in healthy and subclinical mastitic dairy cows. International Journal of Current Microbiology and Applied Sciences, 8(10), 703–710. https://doi.org/10.20546/ijcmas.2019.810.081
[16] Shukla, P. C. and Desai, M. C. (1988). Neem (Azadirachta indica, Juss.) as a source of cattle feed. International Tree Crops Journal, 5(1–2), 135–142. https://doi.org/10.1080/01435698.1988.9752848
[17] Taethaisong, N., Paengkoum, S., Kaewwongsa, W., Onjai-uea, N., Thongpea, S. and Paengkoum, P. (2023). The effect of neem leaf supplementation on growth performance, rumen fermentation, and ruminal microbial population in goats. Animals, 13(5), 890. https://doi.org/10.3390/ani13050890
[18] Terrill, T., Kaplan, R., Larsen, M., Samples, O., Miller, J. and Gelaye, S. (2001). Anthelmintic resistance on goat farms in Georgia: Efficacy of anthelmintics against gastrointestinal nematodes in two selected goat herds. Veterinary Parasitology, 97(3–4), 261–268. https://doi.org/10.1016/s0304-4017(01)00417-4
[19] Waller, P. J. (1997). Sustainable helminth control of ruminants in developing countries. Veterinary Parasitology, 71(2–3), 195–207. https://doi.org/10.1016/s0304-4017(97)00032-0
[20] Wylie, M. R. and Merrell, D. S. (2022). The antimicrobial potential of the neem tree Azadirachta indica. Frontiers in Pharmacology, 13, 891535. https://doi.org/10.3389/fphar.2022.891535