IJFAF | Determination of the Prevalence of Bluetongue Disease in Goats in Siirt Province by the ELISA Method

Indexing and Abstracting

Determination of the Prevalence of Bluetongue Disease in Goats in Siirt Province by the ELISA Method

Ozgur Yasar CELIK , Tekin ŞAHİN , Gülşah AKGÜL

Download | Downloads : 6 | Total View : 1722

DOI: 10.22161/ijfaf.2.6.1

Journal : International Journal Of Forest, Animal And Fisheries Research(IJFAF)

Abstract:

Bluetongue disease is a disease caused by the bluetongue virus in the Orbivirus genus of the Reoviridae family. The disease can be transmitted by ticks, sperm and vertically but is mainly spread by the Culicoides type flies. While the disease is naturally observed in cattle and sheep, it is more rarely observed in goats. This study was carried out to determine the presence of bluetongue infection in goats raised in Siirt province serologically and to obtain information about its prevalence in the region. The material of the study consisted of a total of 372 goats over one year of age raised in Siirt province. For laboratory analyses, 5 ml blood samples were taken from the jugular vein of goats to tubes without anticoagulant. The samples were centrifuged at 3000 rpm for 10 minutes, and the sera were transferred to Eppendorf tubes and stored at -20 °C until analysis. The serum samples were analyzed by the ELISA (Thermo Scientific Multiskan Go) method using the commercial test kit (Bluetongue Virus VP7 Ab Test Kit, IDEXX). The results were evaluated as positive and negative. Of the 372 goats constituting the material of the study, 252 (67.74%) were seropositive and 120 (32.26%) were seronegative. As a result, in addition to measures for quarantine and vector control in the fight against the disease, it was concluded that vaccines specific to virus serotypes should be used and comprehensive and planned studies should be carried out in relation to the disease in the region and the country.

Keywords:

Bluetongue, ELISA, Goat, Siirt, Turkey

References:

[1] Alpay, G., Tuncer, P., and Yeşilbağ, K. (2014). Serological distribution of some viral infections in catt. Ankara Üniv Vet Fak Derg, 61, 43-48.
[2] Bulut, O., Yavru, S., Yapkic, O., Simsek, A., Kale, M., and Avci, O. (2006). Serological investigation of bluetongue virus infection by serum neutralization test and ELISA in sheep and goats. Bulletin-Veterinary Institute In Pulawy, 50 (3), 305.
[3] Darpel, K.E., Batten, C.A., Veronesi, E., Williamson, S., Anderson, P., Dennison, M., Clifford, S., Smith, C., Philips, L., and Bidewell, C. (2009). Transplacental transmission of bluetongue virus 8 in cattle, UK. Emerging infectious diseases, 15 (12), 2025.
[4] De, A., Batabyal, S., Biswas, S.K., Chand, K., Singh, R.K., and Mondal, B. (2009). Surveillance of bluetongue virus antibody in goats using a recombinant VP7-based indirect ELISA in the coastal saline area of West Bengal, India. Vet Ital, 45 (2), 339-346.
[5] Di Ventura, M., Tittarelli, M., Semproni, G., Bonfini, B., Savini, G., Conte, A., and Lika, A. (2004). Serological surveillance of bluetongue virus in cattle, sheep and goats in Albania. Vet. Ital, 40 (3), 101-104.
[6] García, I., Napp, S., Casal, J., Perea, A., Allepuz, A., Alba, A., Carbonero, A., and Arenas, A. (2009). Bluetongue epidemiology in wild ruminants from Southern Spain. European Journal of Wildlife Research, 55 (2), 173.
[7] Gizaw, D., Sibhat, D., Ayalew, B., and Sehal, M. (2016). Sero-prevalence study of bluetongue infection in sheep and goats in selected areas of Ethiopia. Ethiopian Veterinary Journal, 20 (1), 105-114.
[8] Kulaç, E., Kırmızıgül, A.H., and Yıldıım, Y. (2016). Seroprevalance of Bluetongue Infection in Cattle in Rize Region. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 11 (2), 151-158.
[9] Luo, H., Li, K., Zhang, H., Lan, Y., Peng, J., Shahzad, M., and Wang, J. (2017). Seroprevalence of bluetongue virus infection in goats in the central China. Tropical Biomedicine, 34 (1), 80-83.
[10] Mahmoud, M., and Khafagi, M.H. (2014). Seroprevalence of bluetongue in sheep and goats in Egypt. Vet. World, 7 (4), 205-208.
[11] Mellor, P., and Wittmann, E. (2002). Bluetongue virus in the Mediterranean Basin 1998–2001. The Veterinary Journal, 164 (1), 20-37.
[12] Meteoroloji. (2018). Turkish State Meteorological Service. Retrieved from https://goo.gl/ud7dBK
[13] Mozaffari, A.A., Khalili, M., and Sabahi, S. (2014). High seroprevalence of bluetongue virus antibodies in goats in southeast Iran. Asian Pacific journal of tropical biomedicine, 4, S275-S278.
[14] Mulabbi, E.N., Ayebazibwe, C., Majalija, S., Batten, C.A., and Oura, C.A. (2013). Circulation of bluetongue virus in goats in the Karamoja region of Uganda. Journal of the South African Veterinary Association, 84 (1), 00-00.
[15] Nongdhar, R., Isore, D.P., Joardar, S.N., Samanta, I., Batabyal, K., Dey, S., Lodh, C., and Baruı, A. (2017). Seroprevalence of Bluetongue in Sheep Goats and Cattle of Meghalaya. Indian Journal of Animal Health, 56 (2), 197-202.
[16] Ozan, E., Turan, H.M., Albayrak, H., and Cavunt, A. (2012). Serological determination of pestivirus, bluetongue virus and peste des petits ruminants virus in small ruminants in Samsun province of Turkey. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 7 (1), 27-33.
[17] Saltık, H.S., and Kale, M. (2017). Bluetongue Virus Disease. MAKÜ Sag. Bil. Enst. Derg, 5 (1), 32-44.
[18] Saygılı, R. (2015). Maps. Retrieved from https://goo.gl/mtcJdG
[19] TUIK. (2017). Turkish Statistical Institute. Retrieved from http://www.tuik.gov.tr
[20] Uslu, U., and Dik, B. (2004). Seasonal Distribution of Species Culicoides (Diptera: Ceratopogonidae) in Konya Province. Vet.Bil.Derg., 20 (4), 5-10.
[21] Yeşilbağ, K., Alpay, G., and Karakuzulu, H. (2011). A serologic survey of viral infections in captive ungulates in Turkish zoos. Journal of Zoo and Wildlife Medicine, 42 (1), 44-48.
[22] Yilmaz, V., Yildirim, Y., and Coşkun, N. (2015). Serological Investigation of Bluetongue Virus and Rift Valley Fever Virus Infections in Sheep in Kars Province of Turkey. Van Veterinary Journal, 26 (3), 119-122.