• editor.aipublications@gmail.com
  • Track Your Paper
  • Contact Us
  • ISSN: 2456-866X

International Journal Of Chemistry, Mathematics And Physics(IJCMP)

Phytochemical Investigation and Characterization on the Stem Bark Extract of Croton macrostachyus

Teshale Ayano Begeno , Yonas Mathewos Abose

International Journal of Chemistry, Mathematics And Physics(IJCMP), Vol-4,Issue-6, November - December 2020, Pages 113-121 , 10.22161/ijcmp.4.6.2

Download | Downloads : 8 | Total View : 1004

Article Info: Received: 15 Oct 2020; Received in revised form: 30 Oct 2020; Accepted: 04 Nov 2020; Available online: 19 Dec 2020


Croton macrostachyus which is called ‘rush foil’ or ‘broadleaved croton is a multipurpose, medium sized, drought-deciduous pioneer tree. It is a tall tree found in tropical regions of Africa. The genus Croton belongs to the family Euphorbiaceae, which commonly known asthe ‘spurge’ family, and it is known as ‘Bisana’ (in Amharic).Traditionally, C. macrostachyus used for treatment of malaria, rabies, gonorrhea, wound, diarrhea, hepatitis, jaundice, scabies, toothache, abdominal pain, cancer, typhoid, pneumonia and gastrointestinal disorders and as ethno-veterinary medicine.The air dried and powdered plant material (400g) was first soaked with 500mL n-hexane for 48hours and yielded 2g of n-hexane extract. Marc was soaked with 500mL of chloroform for 36hours and afforded 3.5g of chloroform extract. Finally, Marc was soaked with 500mL of methanol and yielded 18g of methanol extract.The chloroform extract of the stem bark ofC. Macrostachyus afforded a compound coded as EO. Its Structural determination was accomplished by means of spectroscopic techniques, namely IR, 1H NMR,13C NMR and DEPT-135. The compound, EOwas isolated and characterized from the stem bark of C. macrostachyus. Generally, more advanced chromatographic techniques are required to isolate more compounds from stem bark of C. macrostachyus. Also MS and 2D NMR spectroscopic techniques are needed to fully characterize the isolated compound.

C. macrostachyus; ethno-veterinary; characterization; chromatographic techniques; spectroscopic techniques.

[1] John Willey and Sons Ltd. Chichester, (1996): Cotton CM. Ethnobotany: Principles and Applications, 242.
[2] Martin GJ, (1995):Ethnobotany: A Method Manual, Chapman and Hall, London.
[3] A. R. Smith, (1987): “Euphorbaceae,” in Flora of Tropical East Africa, R. M. Polhill, Ed., AABalkema, Rotterdam, TheNetherlands, pp. 20–391.
[4] M. G. Gilbert, (1995):“Flora of Ethiopia and Eritrea,” in Euphorbiaceae,S. Edwards, T. Mesfn, and I. Hedberg, Eds.,AddisAbaba University, Addis Ababa, Ethiopia, pp. 265–380.
[5] K. Wakjira and L. Negash, (2013): “Germination responses of Crotonmacrostachyus (Euphorbiaceae) to various physico-chemicalpretreatment conditions,” South African Journal of Botany, vol. 87, pp. 76–83.
[6] A.B. Tesemma, A. Birnie, and B. Tengnas, (1993):“Useful treesand shrubs for ethiopia: identifcation, propagation and management for agricultural and pastoral communities,” Technical Handbook no. 5, Regional Soil Conservation Unit/SIDA,Regional Soil Conservation Unit/SIDA, Nairobi, Kenya.
[7] D. A. Hines and K. Eckman, (1993):Indigenous Multipurpose Trees forTanzania: Uses and Economic Benefts to thePeople, Food and Agriculture Organization of the United Nations, Rome, Italy.
[8] P. Maundu and B. Tengnas, (2005):Useful Trees and Shrubs for Kenya, World Agroforestry Centre, East and Central Africa RegionalProgramme (ICRAF-ECA), Technical Handbook 35, Nairobi,Kenya.
[9] J. Dechasa, “Influence of Croton macrostachyus on maize yield:Traditional inter-crop farming system,” Walia, vol. 1998,no. 20,p. pp, 1999.
[10] Negash L. (2010): A Selection of Ethiopia's Indigenous Trees: Biology, Uses and Propagation Techniques. Addis Ababa University Press, Addis Ababa, Ethiopia pp 386.
[11] Karunamoorthi K. and Ilango K. (2010): Larvicidal activity of Cymbopogoncitratus (DC) and Croton macrostachyus against Anopheles arabiensis Patton, a potent malaria vector. Eur Rev Med PharmacolSci; 14:57-62.
[12] Salatino A., Salatino M.L.F. and Giuseppina N.J., (2007): Braz. Chem. Soc. pp18, 11.
[13] Bum E.N., Ngah E., Mune R.M.N., ZeMinkoulou D.M., Talla E., Moto F.C.O., Ngoupaye G.T., Taiwe G.S., Rakotonirina A. and Rakotonirina S.V. (2012): Epilepsy Behav. 24: 319.
[14] Friis I. (1992): Forests and forest trees of northeast tropical Africa. Their natural habitats and distribution patterns in Ethiopia, Djibouti and Somalia. Kew Bulletin, Additional Series, XV. Her Majesty's Stationery Office, London pp.396.
[15] Kapingu C.M., Guillaume D., Mbwambo H.Z., Moshi J.M., Uliso .C.F. and Mahunnah A.L.R (2000): Diterpenoids from the roots of Croton macrostachys. Phytochem.,54:767–770.
[16] Orwa C., Mutua A., Kindt R., Jamnadass R. and Simons A. (2009): Agroforestry Database: a tree reference and selection guide version. pdf accessed on 2 April 2015.
[17] Stuart KL., and Graham L.,(1973): Alkaloids Biosynthesis in croton Flavens. Phytochemistry.12:1067-1072.
[18] Tolossa K, Debela E, Athanasiadou S, Tolera A, Ganga G, Houdijk J. Ethno-medicinal study of plants used for treatment of human and livestock ailments by traditional healers in South Omo, Southern Ethiopia. J EthnobiolEthnomed.2013; 9(1): 32.
[19] Carlet J., Jarlier V., Harbarth S., Voss A., Goossens H., Pittet D., (2012): Ready for a world without antibiotics? The pensieres antibiotic resistance calls to action. Antimicrob Resist Infect Control; 1: 11.
[20] Khameneh B, Diab R, Ghazvini K, Bazzaz B.,(2016): Breakthroughsin bacterial resistance mechanisms and the potential ways tocombat them. MicrobPathog.; 95: 32-42.
[21] Kiranmayi ChB, Krishnaiah N, Mallika EN. Escherichia coli O157:H7-An emerging pathogen in foods of animal origin. Vet World. 2010; 3(8): 382-389.
[22] Krishnaiah D., Sarbatly R., Bono A., (2007): Phytochemical antioxidants for health and medicine: A move towards nature. Biotechnology Molecular Biology;1: 97-104.
[23] Lai PK., Roy J., (2004): Antimicrobial and chemo preventive properties of herbs and species. Curr Med Chem.; 11: 1451-1460.
[24] Lee JH., (2003): Methicillin (oxacillin)-resistant Staphylococcus aureusstrains isolated from major food animals and their potential transmission to humans. App Environ Microbiol.;69(11): 6489-6494.
[25] Le Jeune JT., Besser TE., Hancock DD.,(2001):Cattle water troughs asreservoirs of Escherichia coli O157. Appl Environ Microbiol; 67.